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KMID : 1094720100150010145
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Biotechnology and Bioprocess Engineering
2010 Volume.15 No. 1 p.145 ~ p.151
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Fabrication of Protein Microarrays Using the Electrospray Deposition (ESD) Method: Application of Microfluidic Chips in Immunoassay
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Lee Bum-Hwan
Tajima Akihiko
Nagamune Teruyuki
Kim Joon-Wan
Yamagata Yutaka
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Abstract
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In this study, antibody-based protein microarrays for high-throughput immunoassay were fabricated on an aldehydemodified indium-tin oxide glass plate using the electrospray deposition (ESD) method and their characteristics were evaluated immunochemically. The microarrays were also integrated into microfluidic chips with a polydimethylsiloxane (PDMS) micro-channel to detect human cytokines, which were quantitatively analyzed with a high resolution chargecoupled device. Simultaneous detection of various antigens was performed using the microarrays with considerable sensitivity (ca. 100 pg/mL). The results of this study indicate that microfluidic chip comprising a protein microarray formed by the ESD method and a PDMS micro-channel could be easy to handle, and offers high-throughput detection of molecular biomarkers.
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KEYWORD
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electrospray deposition method, protein microarray, biochip, high-throughput Immunoassay
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